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1

Production of recombinant protein therapeutics in cultivated mammalian cells

Wurm, Florian M

Journal:

Nature Biotechnology

Year:

2004

Language:

english

File:

PDF, 235 KB

english, 2004

2

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Zagari, Francesca, Jordan, Martin, Stettler, Matthieu, Broly, Hervé, Wurm, Florian M.

Journal:

New Biotechnology

Year:

2013

Language:

english

File:

PDF, 910 KB

english, 2013

3

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

Blessing, Daniel, Vachey, Gabriel, Pythoud, Catherine, Rey, Maria, Padrun, Vivianne, Wurm, Florian M., Schneider, Bernard L., Déglon, Nicole

Journal:

Molecular Therapy — Methods & Clinical Development

Year:

2019

Language:

english

File:

PDF, 2.37 MB

english, 2019

4

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

Gaurav Backliwal, Markus Hildinger, Ivan Kuettel, Fanny Delegrange, David L. Hacker, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2008

Language:

english

File:

PDF, 198 KB

english, 2008

5

TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology

Maria J De Jesus, Philippe Girard, Michaela Bourgeois, Gwendoline Baumgartner, Barbara Jacko, Hanspeter Amstutz, Florian M Wurm

Journal:

Biochemical Engineering Journal

Year:

2004

Language:

english

File:

PDF, 334 KB

english, 2004

6

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

Lucia Baldi, David L. Hacker, Myriam Adam, Florian M. Wurm

Journal:

Biotechnology Letters

Year:

2007

Language:

english

File:

PDF, 152 KB

english, 2007

7

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Mattia Matasci, David L. Hacker, Lucia Baldi, Florian M. Wurm

Journal:

Drug Discovery Today: Technologies

Year:

2008

Language:

english

File:

PDF, 433 KB

english, 2008

8

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

Zhu, Li-kuan, Song, Bo-yan, Wang, Zhen-long, Monteil, Dominique T., Shen, Xiao, Hacker, David L., De Jesus, Maria, Wurm, Florian M.

Journal:

Biotechnology Progress

Year:

2017

Language:

english

File:

PDF, 495 KB

english, 2017

9

Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells

Petra Meissner, Horst Pick, Alexandra Kulangara, Philippe Chatellard, Kirstin Friedrich, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2001

Language:

english

File:

PDF, 1.39 MB

english, 2001

10

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

Gaurav Backliwal, Markus Hildinger, Vivek Hasija, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2008

Language:

english

File:

PDF, 173 KB

english, 2008

11

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Stéphanie Tissot, Agata Oberbek, Martino Reclari, Matthieu Dreyer, David L. Hacker, Lucia Baldi, Mohamed Farhat, Florian M. Wurm

Journal:

New Biotechnology

Year:

2011

Language:

english

File:

PDF, 538 KB

english, 2011

12

Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L

Xiaowei Zhang, Cédric-Alain Bürki, Matthieu Stettler, Dario De Sanctis, Marco Perrone, Marco Discacciati, Nicola Parolini, Maria DeJesus, David L. Hacker, Alfio Quarteroni, Florian M. Wurm

Journal:

Biochemical Engineering Journal

Year:

2009

Language:

english

File:

PDF, 1.66 MB

english, 2009

13

Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors

Stéphanie Tissot, Mohamed Farhat, David L. Hacker, Tibor Anderlei, Markus Kühner, Christos Comninellis, Florian Wurm

Journal:

Biochemical Engineering Journal

Year:

2010

Language:

english

File:

PDF, 434 KB

english, 2010

14

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Yashas Rajendra, Divor Kiseljak, Lucia Baldi, David L Hacker, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 119 KB

english, 2011

15

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Xiaowei Zhang, Isabel Fernandez Garcia, Lucia Baldi, David L. Hacker, Florian M. Wurm

Journal:

Biotechnology Letters

Year:

2010

Language:

english

File:

PDF, 254 KB

english, 2010

16

Transient Gene Expression in Suspension HEK-293 Cells: Application to Large-Scale Protein Production

Lucia Baldi, Natalie Muller, Sylviane Picasso, Raymond Jacquet, Philippe Girard, Huy Phan Thanh, Elisabeth Derow, Florian M. Wurm

Journal:

Biotechnology Progress

Year:

2005

Language:

english

File:

PDF, 125 KB

english, 2005

17

Biological properties of a CD4 immunoadhesin

Byrn, Randal A., Mordenti, Joyce, Lucas, Catherine, Smith, Douglas, Marsters, Scot A., Johnson, Jennifer S., Cossum, Paul, Chamow, Steven M., Wurm, Florian M., Gregory, Timothy

Journal:

Year:

1990

File:

PDF, 465 KB

1990

18

Extraction of plasmid DNA using reactor scale alkaline lysis and selective precipitation for scalable transient transfection

Jason L. Wright, Martin Jordan, Florian M. Wurm

Journal:

Year:

2001

Language:

english

File:

PDF, 499 KB

english, 2001

19

Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors

Wolf Klöckner, Stéphanie Tissot, Florian Wurm, Jochen Büchs

Journal:

Biochemical Engineering Journal

Year:

2012

Language:

english

File:

PDF, 776 KB

english, 2012

20

Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery

Martin Bertschinger, Gaurav Backliwal, Arnaud Schertenleib, Martin Jordan, David L. Hacker, Florian M. Wurm

Journal:

Journal of Controlled Release

Year:

2006

Language:

english

File:

PDF, 783 KB

english, 2006

21

kLa as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Stéphanie Tissot, Patrik O. Michel, David L. Hacker, Lucia Baldi, Maria De Jesus, Florian M. Wurm

Journal:

New Biotechnology

Year:

2012

Language:

english

File:

PDF, 1.00 MB

english, 2012

22

Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

Kadlecova, Zuzana, Baldi, Lucia, Hacker, David, Wurm, Florian Maria, Klok, Harm-Anton

Journal:

Biomacromolecules

Year:

2012

Language:

english

File:

PDF, 591 KB

english, 2012

23

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Ehrmann, Klaus, Saillen, Nicolas, Vincent, Franck, Stettler, Matthieu, Jordan, Martin, Wurm, Florian Maria, Besse, Pierre-Andr?, Popovic, Radivoje

Journal:

Year:

2007

Language:

english

File:

PDF, 745 KB

english, 2007

24

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Monteil, Dominique T., Shen, Xiao, Tontodonati, Giulia, Baldi, Lucia, Hacker, David L., Wurm, Florian M.

Journal:

Analytical Biochemistry

Year:

2016

Language:

english

File:

PDF, 437 KB

english, 2016

25

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Monteil, Dominique T., Juvet, Valentin, Paz, Jonathan, Moniatte, Marc, Baldi, Lucia, Hacker, David L., Wurm, Florian M.

Journal:

Biotechnology Progress

Year:

2016

Language:

english

File:

PDF, 1.56 MB

english, 2016

26

463. Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors

Blessing, Daniel R., Schneider, Bernard, Wurm, Florian M., Déglon, Nicole

Journal:

Molecular Therapy

Year:

2016

Language:

english

File:

PDF, 340 KB

english, 2016

27

Anatomical locations in the oral cavity where surgical resections of oral squamous cell carcinomas are associated with a close or positive margin—a retrospective study

Kerker, Florian Alexander, Adler, Werner, Brunner, Kathrin, Moest, Tobias, Wurm, Matthias C., Nkenke, Emeka, Neukam, Friedrich Wilhelm, von Wilmowsky, Cornelius

Journal:

Clinical Oral Investigations

Year:

2018

Language:

english

File:

PDF, 397 KB

english, 2018

28

Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion

Wurm, Florian, Wurm, Maria

Journal:

Year:

2017

Language:

english

File:

PDF, 1.69 MB

english, 2017

29

Analysis of volumetric mass transfer coefficient (kLa) in small- (250Â mL) to large-scale (2500 L) orbitally shaken bioreactors

Zhu, Likuan, Xu, Bin, Wu, Xiaoyu, Lei, Jianguo, Hacker, David L., Liang, Xiong, Wurm, Florian M.

Journal:

Year:

2020

File:

PDF, 1.04 MB

2020

30

Fluorescent proteins in animal cells for process development: Optimization of sodium butyrate treatment as an example

Lisa Hunt, Pascal Batard, Martin Jordan, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2002

Language:

english

File:

PDF, 209 KB

english, 2002

31

Serum-free large-scale transient transfection of CHO cells

Madiha Derouazi, Philippe Girard, Frédéric Van Tilborgh, Keyvan Iglesias, Natalie Muller, Martin Bertschinger, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2004

Language:

english

File:

PDF, 1.02 MB

english, 2004

32

Low-temperature pausing of cultivated mammalian cells

Lisa Hunt, David L. Hacker, Frédéric Grosjean, Maria De Jesus, Lorenz Uebersax, Martin Jordan, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2005

Language:

english

File:

PDF, 545 KB

english, 2005

33

Orbital shaker technology for the cultivation of mammalian cells in suspension

Natalie Muller, Philippe Girard, David L. Hacker, Martin Jordan, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2005

Language:

english

File:

PDF, 283 KB

english, 2005

34

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Matthieu Stettler, Nicolas Jaccard, David Hacker, Maria De Jesus, Florian M. Wurm, Martin Jordan

Journal:

Biotechnology and Bioengineering

Year:

2006

Language:

english

File:

PDF, 143 KB

english, 2006

35

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Sebastien Chenuet, Danielle Martinet, Nathalie Besuchet-Schmutz, Marianne Wicht, Nicolas Jaccard, Anne-Charlotte Bon, Madiha Derouazi, David L. Hacker, Jacques S. Beckmann, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2008

Language:

english

File:

PDF, 260 KB

english, 2008

36

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

Agata Oberbek, Mattia Matasci, David L. Hacker, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2011

Language:

english

File:

PDF, 640 KB

english, 2011

37

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

Mattia Matasci, Lucia Baldi, David L. Hacker, Florian M. Wurm

Journal:

Biotechnology and Bioengineering

Year:

2011

Language:

english

File:

PDF, 434 KB

english, 2011

38

A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles

Martin Bertschinger, Sophie Chaboche, Martin Jordan, Florian M. Wurm

Journal:

Analytical Biochemistry

Year:

2004

Language:

english

File:

PDF, 146 KB

english, 2004

39

Polyethylenimine-based quality control assay for plasmid DNA

Martin Bertschinger, Cédric Burki, Gaurav Backliwal, David L. Hacker, Martin Jordan, Florian M. Wurm

Journal:

Analytical Biochemistry

Year:

2006

Language:

english

File:

PDF, 151 KB

english, 2006

40

A collagen-poly(lactic acid-co-ɛ-caprolactone) hybrid scaffold for bladder tissue regeneration

Eva-Maria Engelhardt, Lionel A. Micol, Stephanie Houis, Florian M. Wurm, Jöns Hilborn, Jeffrey A. Hubbell, Peter Frey

Journal:

Year:

2011

Language:

english

File:

PDF, 1.24 MB

english, 2011

41

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Sarah Wulhfard, Lucia Baldi, David L. Hacker, Florian Wurm

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 290 KB

english, 2010

42

A simple high-yielding process for transient gene expression in CHO cells

Yashas Rajendra, Divor Kiseljak, Lucia Baldi, David L. Hacker, Florian M. Wurm

Journal:

Journal of Biotechnology

Year:

2011

Language:

english

File:

PDF, 469 KB

english, 2011

43

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Gaurav Backliwal, Markus Hildinger, Sebastien Chenuet, Maria DeJesus, Florian M. Wurm

Journal:

New Biotechnology

Year:

2008

Language:

english

File:

PDF, 402 KB

english, 2008

44

Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells

Isabelle Bisson, Marek Kosinski, Sylvie Ruault, Bhuvanesh Gupta, Jöns Hilborn, Florian Wurm, Peter Frey

Journal:

Year:

2002

Language:

english

File:

PDF, 330 KB

english, 2002

45

Large-scale transient expression in mammalian cells for recombinant protein production

Florian Wurm, Alain Bernard

Journal:

Current Opinion in Biotechnology

Year:

1999

Language:

english

File:

PDF, 70 KB

english, 1999

46

CHO cell lines generated by PiggyBac transposition

Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 157 KB

english, 2011

47

Transient transfection of insect Sf-9 cells in TubeSpin®bioreactor 50 tubes

Xiao Shen, Patrik O Michel, Qiuling Xie, David L Hacker, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 140 KB

english, 2011

48

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Sowmya Balasubramanian, Mattia Matasci, Lucia Baldi, David L Hacker, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 144 KB

english, 2011

49

Rapid recombinant protein production from pools of transposon-generated CHO cells

Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 207 KB

english, 2011

50

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin®bioreactors

João Pereira, Yashas Rajendra, Lucia Baldi, David L Hacker, Florian M Wurm

Journal:

BMC Proceedings

Year:

2011

Language:

english

File:

PDF, 241 KB

english, 2011